How to re-create IIS Virtual Directories
When creating Virtual Directories for BizTalk solutions with HTTP front end, the BizTalk WCF Service Publishing Wizard is used.
This is fine, but usually you will need to move the generated web site to your solution.
Even if it is deleted and IIS restarted this can still cause issues.
One work-around is to rename the site to something else.
Or you can delete this file: